Move to content body Move to content menus

SEOUL NATIONAL UNIVERSITY

  • home
  • sitemap
  • korean
search

RESEARCH

 

Research Highlights

Professor KIM Jin-Soo’s RNA-Guided Genome Editing Using Digenome-seq

  • February 9, 2015
  • Hit 10736
    • facebook
    • twitter
    • print

Professor KIM Jin-Soo (Dept. of Chemistry)
Professor KIM Jin-Soo

Professor Kim Jin-Soo’s research team (Department of Chemistry) has announced Digenome-seq -- in vitro Cas9-digested whole-genome sequencing -- to profile genome-wide Cas9 off-target effects in human cells. Using Digenome-seq, the team successfully proved that CRISPR-Cas9, an RNA-guided genome editing system, works effectively and specifically on the target genome. The team also found that Cas9 off-target effects can be avoided by replacing 'promiscuous' single guide RNAs (sgRNAs) with modified sgRNAs. Professor Kim wrote that “Digenome-seq is a robust, sensitive, unbiased and cost-effective method for profiling genome-wide off-target effects of programmable nucleases including Cas9.” This research was published online on February 9 in Nature Methods.

Summary by LEE Hee Un, SNU English Editor, gurume0221@snu.ac.kr
Proofread by Melora Brett Briana Johnson, morningcalm2@gmail.com

List of postings
NO. TITLE DATE HIT
155 World’s Smallest Bubbles Observed Mar. 27, 2015 16485
154 New Bell-measurement Scheme to Teleport Photons with High Success Probability Mar. 17, 2015 5798
153 Why Targeted Cancer Therapy Seldom Works in Treating Ovarian Cancer Mar. 10, 2015 7066
152 Professor ZHANG Byoung-Tak Develops Imaginative Computer Program Feb. 25, 2015 11806
151 Professor KWON Sunghoon Develops DNA Laser Printer Feb. 24, 2015 9196