Move to content body Move to content menus

SEOUL NATIONAL UNIVERSITY

  • home
  • sitemap
  • korean
search

RESEARCH

 

Research Highlights

Gene-Editing Breakthrough

  • April 12, 2017
  • Hit 7693
    • facebook
    • twitter
    • print

Professor KIM Jin-Soo
Professor KIM Jin-Soo

Professor Kim Jin-Soo and training researcher Kim Daesik, both of the Department of Chemistry, were part of a research team at the Center for Genomic Engineering, within the Institute for Basic Science (IBS), that proved that a newly developed gene editing technique will be helpful in combatting genetic mutation that causes diseases such as cystic fibrosis, sickle cell anemia, and color blindness.

The field of biology has been buzzing with the latest developments of gene editing tools. The most current tool is called CRISPR -- third-generation DNA “scissors” that are less expensive and more efficient than previously available editing tools. CRISPR-Cas9 and CRISPR-Cpf1 edit out faulty DNA sequences resulting from random DNA deletions and insertions.

Last year, scientists figured out a way to substitute and correct just one of the four basic nucleotides: Adenine (A), Cytosine (C), Guanine (G), and thymine (T), whereas previous tools could not modify the strand at such a fine level. This discovery represents a big leap forward for the scientific community, considering that several critical diseases -- such as cystic fibrosis, sickle cell anemia, and color blindness -- are caused by point mutations, and not by random insertion or deletion.

This new base-editing scissors was a variation of the existing CRISPR-Cas9, but fused with an enzyme called cytosine deaminase, which would replace C with T. Despite the excitement of this new development, scientists were still unsure if it was applicable to the gene that they were targeting, or if it was functioning outside of the DNA bases.

The team headed by Professor Kim proved the accuracy of the tool by modifying and using an error-checking technique called Digenome-seq. In the process, they also improved the computer program (Digenome 2.0) to detect “off-target” operations (if the editing tool strays from the genome, it is called off-target). The program also compared different guide RNAs which worked to lead the editing tool along the genome to spot the targeted base.

This significant finding was published on the cover of the academic journal Nature Biotechnology.

Written by Ho Jung Annie Hwang, SNU English Editor, annieohan@snu.ac.kr
Reviewed by Professor Travis Smith, Department of Asian Languages and Civilizations, tlsmith@snu.ac.kr

List of postings
NO. TITLE DATE HIT
233 Professor Lee’s New Holographic Microscopes Sept. 4, 2018 7576
232 Artificial Nerves Used for Neurorobotics and Neuroprosthetics May 31, 2018 5444
231 Electronic Skins that Wirelessly Activate Fully Soft Robots May 30, 2018 892
230 SNU Prof. Waldman research team found that the fungus leading world-wide fatal amphibian disease was originated in Korea. May 23, 2018 6151
229 A New Generation of Solar Energy May 1, 2018 3427